The Jamaican defending world champion and world record holder was a victim of a rule in effect since 1 January, 2010 meaning immediate disqualification for a false start.
It was seen as a way to prevent gamesmanship from potentially slower sprinters looking to unsettle their rivals and to enable organisers to stick more closely to TV-dependent schedules.
Other athletes have paid the price but the 'Cult of Bolt' has ensured it is now the major talking point at the worlds in Daegu, South Korea.
Even before the dust has settled over the incident, a British bookmaker offered odds of 5/2 that false start rules will be changed to permit two rather than the present permitted one before the London Olympics in 2012.
But despite the furore and the loss of the globe's most marketable sports star from the 100m final, athletics chiefs confirmed there were no immediate plans to discuss a rule change.
'Rules are rules and those are the rules at the moment,' an IAAF spokesman told AFP. 'There has been no decision in terms of it being brought up at the next council meeting (at the end of the championships) that I know of.'
In a statement issued after Sunday's dramatic events, the IAAF said it was 'disappointed' that Bolt had false-started but that a sport's credibility depended on its rules and they had to be applied consistently and fairly.
The IAAF spokesman reiterated Monday that the Bolt disqualification had showed the essential fairness of the rules as not even athletics' biggest star had been spared.
'Bolt is still running in the 200m and the 4x100m. We need to give it a little bit of time. All of these reactions are very raw,' he said. 'We need to give a little bit of time to reflect.'
Bolt's disqualification shook up his rivals but after a delay of a few minutes team-mate Yohan Blake held his nerve for the restart and powered to victory in 9.92sec.
American Walter Dix was second in 10.08 and 2003 world champion Kim Collins of St Kitts and Nevis won bronze in 10.09.
Blake, Bolt's training partner under coach Glen Mills, admitted to conflicting emotions following his unexpected win.
'I knew I would do this one day but I did not expect it for today. Now I am traumatised and have mixed feelings -- very sad for Usain Bolt but at the same time I am enjoying this moment very much,' he said.
Veteran Collins said: 'As much as I want to be on the podium, tonight is a sad night for athletics.
'I was looking for something to say like there was a malfunction, it didn't happen, let's do it again because he is Olympic champion and Olympic record holder and people all over the world see him tonight, but that didn't happen and it's just really sad.
'I don't think the false start rule is the right one but as the IAAF think it is good for TV it will probably stay,' Collins said.
A shocked Dix described the situation as 'surreal', adding: 'I didn't really think they would kick him (Bolt) out.'
Bolt still has a chance of redemption in the 200m and 4x100m sprints, with injured sprinter Tyson Gay predicting: 'He's going to put on his 2009 face and go to war.' Collins and Blake said he would have a point to prove.
But for the devastated star, who turned 25 last week, it was took early to predict what would happen later in the week, saying he needed time to digest the events of Sunday evening.
Speaking about the defence of his 200m title, which begins on Friday, he said: 'How will I go? It's on a Friday right? Then we'll have to see on Friday.'
Bolt has been the unchallenged star of world sprinting in recent years.
After storming to the 100m and 200m sprint double in then-world record times at the 2008 Olympics, he matched his feat the following year at the Berlin worlds, setting new marks of 9.58 and 19.19sec.
American Trey Hardee retained his world decathlon title on Sunday as he led teammate Ashton Eaton to a US 1-2, with Cuban Leonel Suarez taking bronze.
Hardee amassed 8,607 points in the gruelling 10-discipline event, with Eaton producing the race of his life in the 1500m to take silver by just four points from Suarez (8,501), who had been in second going into the final event.
China's Li Yanfeng won the women's discus title at the World Athletics Championships on Sunday.
Li recorded a best throw of 66.52m, with Germany's Nadine Muller taking silver (65.97), and Cuban Yarelys Barrios claiming bronze (65.73).
Ethiopia's Ibrahim Jeilan produced an amazing home-stretch sprint to trump British favourite Mo Farah at the line of an enthralling men's 10,000m at the world championships on Sunday.
Jeilan was a shock winner after defending champion Kenenisa Bekele, bidding for a fifth consecutive title and unbeaten over the distance, pulled out of the race with 10 laps to go.