Bachchan, who joined Twitter in 2010, is an avid user of the site and has tweeted 11,108 times till date.
The 69-year-old took the number one spot after beating last time winner Priyanka, who now stands second on the list with 1,841,804 followers and 5,693 tweets.
The actress follows 138 people among which are Shahrukh Khan, Big B and Hrithik Roshan.
Cricketer Sachin Tendulkar came third on the list with 1,786,243 followers and Shah Rukh Khan landed on the fourth position with 1,612,989 followers.
Actor Aamir Khan, who has given hits likes 'Taare Zameen Par' and 'Dhobi Ghat', came fifth with 1,506,083 followers on Twitter and 'Dabangg' star Salman Khan ranked sixth with 1,485,203 followers.